Staff Research Associate 3

SRA 3 (009611)

UCPath Position ID: TBD_4081

Position Description History/Status

Approved Date:

3/26/2025 3:04:13 PM

Date Last Edited:

3/26/2025 3:04:09 PM

Last Action Effective Date:

Organization Details

Business Unit (Location):

LACMP

Organization Code:

1600O

Organization:

BASIC BIOMEDICAL SCIENCES

Division Code:

1610D

Division:

BASIC BIOMEDICAL SCIENCES DIV

Department:

230100 - MICROBIOLOGY, IMMUNO & MOLECULAR GENETIC

Position Details

UCPath Position Number:

TBD_4081

Position Description ID

237430

UC Payroll Title:

SRA 3 (009611)

Business Title:

Staff Research Associate 3

Personnel Program

Professional and Support Staff (PSS)

Salary Grade:

STEPS

Job Code FLSA:

Exempt

Union Code (Collective Bargaining Unit):

RX: Research Support Professionals

Employee Relations Code:

E: All Others - Not Confidential

Employee Class (Appt Type):

2 - Staff: Career

Full-Time Equivalent (FTE)

1

SUPERVISION

UCPath Reports to Position Number:

40867983

Reports to Payroll Title:

Assistant Adjunct Professor

UCPath Department Head Position Number:

Sara S. Lee, Ph.D.

Department Head Payroll Title:

Admin Mgr 2

GENERAL SUPERVISION - Indicates that the incumbent develops procedures for performance of variety of duties; or performs complex duties within established policy guidelines.

POSITION SUMMARY

Under the general supervision of the laboratory Principal Investigator and the Assistant Adjunct Professor, the SRA major functions will be to evaluate targeted genome editing in hematopoietic cells. The role will involve independent research on the use of site-specific nucleases, base editors and other techniques to directly modify the human genome in hematopoietic stem cells. The SRA will also be responsible for sharing oversight of one to two SRA lab members, as well as research performed by graduate or undergraduate students and other trainees.

The MIMG department mission is to provide the highest quality research and education possible in the interdisciplinary fields of Microbiology, Immunology and Molecular Genetics. To meet this goal, we work together as scientists, educators and students to identify and address the most relevant problems in microbial pathogenesis and physiology, host cell biology and immune defense, and the host-pathogen interface.

Evaluate Gene Modification of Hematopoietic Stem Cells

1. Design, perform electroporations, and analyze experiments for the genome modifications of cell

lines and hematopoietic stem cells.

2. Design and perform PCR, qPCR, and ddPCR

assays.

3. Perform flow cytometry analysis on modified

cells.

4. Perform data analysis.

5. Maintain supplies and equipment needed for

above duties.

60%

Generate Libraries for Next-Generation Sequencing

1. Design and perform library preparation for high-throughput sequencing of modified cells.

2. Maintain supplies and equipment needed for library preparation.

15%

Supervision

1. Oversee work performed on projects by SRAs and undergraduate and graduate students and trainees.

10%

Perform Routine Lab Support

1. Prepare reagents, maintain equipment and lab environment, maintain necessary certifications.

10%

Molecular Cloning

1. Strong cloning knowledge.

5%

•

Performs other duties as assigned.

•

Complies with all policies and standards.

•

Complies with the University of California, Los Angeles (UCLA) Principles of Community.

•

This position description is not intended to be a complete list of all responsibilities, duties or skills required for the job and is subject to review and change at any time, with or without notice, in accordance with the needs of the organization.

QUALIFICATIONS

Master's Degree

Master's degree or PhD in Biological Sciences.

Preferred

Or

PhD

Master's degree or PhD in Biological Sciences.

Preferred

Tissue Culture

Minimum three years of experience in tissue culture procedures for both cell lines and stem cells; and gene modification techniques in cell lines and primary cells, and molecular analyses.

Required

Ability to work both independently and as part of a team and to establish and maintain cooperative working conditions and relationships with other staff members and students.

Required

Ability to drive projects and carry out experiments and analysis with minimal supervision.

Required

Knowledge of sterile techniques used in tissue culture.

Required

Demonstrated skill with tissue culture procedures for multiple cell types: both cell lines and hematopoietic stem cells.

Required

Strong skills in computing cell concentrations, solution preparation, and other needed mathematical calculations.

Required

Experience with preparing, running, and analyzing cell samples by flow cytometry. Experience with Diva software highly desired.

Required

Advanced molecular biology techniques. Skills in PCR, DNA and RNA isolation and experience in molecular cloning.

Required

Good communication skills (written, oral) to oversee SRAs and trainees, maintain lab protocols, notebooks, and to prepare and make research presentations in lab meetings.

Required

Organizational skills with strong attention to detail and flexibility to manage multiple tasks with competing deadlines;

strong organization skills to maintain detailed laboratory protocols, ordering information, and inventories of reagents.

Required

Knowledge of laboratory safety procedures associated with the handling and disposal of chemicals and chemical waste, and biohazards and biohazardous waste.

Preferred

Maintain a flexible schedule to accommodate non-customary timing constraints of experiments. Ability to work occasional evenings and weekends.

Required

Ability to move and lift up to 25 lbs.

Required

SPECIAL REQUIREMENTS AND/OR CONDITIONS OF EMPLOYMENT

Background Check: Continued employment is contingent upon the completion of a satisfactory background investigation.

Live Scan Background Check: A Live Scan background check must be completed prior to the start of employment.

Age Requirement: Candidate(s) must be 18 years or older to be eligible to be hired.

LOCATION AND PHYSICAL, ENVIRONMENTAL, MENTAL (PEM) REQUIREMENTS

Environment and Work Location Information

Environment Type:

Non-Clinical Setting

Location Setting:

Campus

Location:

Dept of MIMG

Standing/Walking

X

X

Sitting

X

X

Bending/Stooping

X

X

Squatting/Kneeling

X

X

Climbing

X

Lifting/Carrying/Push/Pull 0-25 lbs

X

X

Lifting/Carrying/Push/Pull 26-50 lbs

X

Lifting/Carrying/Push/Pull over 50 lbs

X

Physical requirements other

X

Chemicals, dust, gases, or fumes

X

X

Loud noise levels

X

Marked changes in humidity or temperature

X

Microwave/Radiation

X

Operating motor vehicles and/or equipment

X

Exposures other

X

Sustained attention and concentration

X

X

Complex problem solving/reasoning

X

X

Ability to organize & prioritize

X

X

Communication skills

X

X

Numerical skills

X

X

Mental demands other

X

X

Other Mental Requirements

If "Mental demands other" was selected above, please explain:

X

Classification 2: Position in which required tasks normally do not involve exposure to blood, body fluids or tissues, but may require performing unplanned Classification 1 tasks. In these jobs the normal work performance involves no exposure to blood, body fluids, or tissues. However, exposure or potential exposure may be required as a condition of employment.